Use of the complete genome sequence information of Haemophilus influenzae strain Rd to investigate lipopolysaccharide biosynthesis

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TypeArticle
Journal titleMol.Microbiol.
Volume22
Issue5
Pages951965; # of pages: 15
Subjectanalogs & derivatives; analysis; Animals; Animals,Newborn; Antibodies; antibody; biosynthesis; chemistry; Chromosome Mapping; Cloning,Molecular; database; Electrophoresis,Polyacrylamide Gel; Electrospray; Fractionation; GENE; Genes; genetics; Genome,Bacterial; Glycine; Haemophilus; Haemophilus influenzae; HAEMOPHILUS-INFLUENZAE; Human; Immunoblotting; immunology; Infant; LIPOPOLYSACCHARIDE; Lipopolysaccharides; LPS; mass spectrometry; MASS-SPECTROMETRY; metabolism; Models,Molecular; MONOCLONAL-ANTIBODIES; MONOCLONAL-ANTIBODY; Monte Carlo Method; Mutagenesis; MUTANT; pathogenicity; POTENTIAL; Rats; Role; SEQUENCE; SEQUENCES; Spectrum Analysis,Mass; STRAIN; STRAINS; structure; Support,Non-U.S.Gov't; Virulence
AbstractThe availability of the complete 1.83-megabase-pair sequence of the Haemophilus influenzae strain Rd genome has facilitated significant progress in investigating the biology of H.influenzae lipopolysaccharide (LPS), a major virulence determinant of this human pathogen. By searching the H. influenzae genomic database, with sequences of known LPS biosynthetic genes from other organisms, we identified and then cloned 25 candidate LPS genes. Construction of mutant strains and characterization of the LPS by reactivity with monoclonal antibodies, PAGE fractionation patterns and electrospray mass spectrometry comparative analysis have confirmed a potential role in LPS biosynthesis for the majority of these candidate genes. Virulence studies in the infant rat have allowed us to estimate the minimal LPS structure required for intravascular dissemination. This study is one of the first to demonstrate the rapidity, economy and completeness with which novel biological information can be accessed once the complete genome sequence of an organism is available
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberHOOD1996
NPARC number9388930
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Record identifier25104f90-9ffb-4cb3-9eab-17b0d58a0ab4
Record created2009-07-10
Record modified2016-05-09
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