Epidermal growth factor tethered through coiled-coil interactions induces cell surface receptor phosphorylation

Download
  1. Get@NRC: Epidermal growth factor tethered through coiled-coil interactions induces cell surface receptor phosphorylation (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1021/bc9001147
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleBioconjugate Chemistry
Volume20
Issue8
Pages15691577; # of pages: 9
SubjectAnimal; bio; Biotechnology; Epidermal Growth Factor; Phosphorylation; Protein; Proteins; Tissue Engineering
AbstractWe have elaborated and validated a novel approach for the oriented tethering of proteins such as the epidermal growth factor (EGF) on aminated surfaces. The grafting reactions were optimized to generate a dense and homogeneous EGF layer. Impact of EGF orientation on A-431 cellular response was investigated. Our results demonstrate that, in sharp contrast to responses obtained with soluble EGF supply or with randomly grafted EGF, oriented immobilization of EGF via a de novo designed coiled-coil capture system leads to a sustained phosphorylation of A-431 cell surface EGF receptors. Our results thus indicate that oriented protein immobilization via coiled-coil interactions is an efficient and versatile method to control tethering of bioactive molecules for future applications in the field of regenerative medicine and tissue engineering.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Biotechnology Research Institute
Peer reviewedYes
NRC number52736
NPARC number14021379
Export citationExport as RIS
Report a correctionReport a correction
Record identifier25695f56-bcf0-4d5b-ab48-f60592a19e6c
Record created2010-06-02
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)