Stabilized recombinant suppressors of RNA silencing: functional effects of linking monomers of Carnation Italian Ringspot virus p19

Download
  1. Get@NRC: Stabilized recombinant suppressors of RNA silencing: functional effects of linking monomers of Carnation Italian Ringspot virus p19 (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1016/j.bbapap.2007.09.014
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleBiochimica et Biophysica Acta (BBA): Proteins and Proteomics
ISSN0006-3002
Volume1774
Issue12
Pages15281535; # of pages: 8
SubjectAmino Acid Sequence; Cloning, Molecular; Dimerization; Genes, Suppressor; Models, Molecular; Protein Binding; Protein Conformation; Protein Denaturation; Protein Engineering; Recombinant Fusion Proteins; RNA Interference; Tombusvirus; Viral Core Proteins
AbstractEukaryotes have evolved complex cellular responses to double-stranded RNA including the RNA silencing pathway. Tombusviruses have adapted a mechanism to evade RNA silencing that involves a 19 kDa dimeric protein (p19) that is a suppressor of RNA silencing. In order to develop stabilized p19 proteins, linked versions of p19 from the Carnation Italian Ringspot virus (CIRV) were constructed that joined the C-terminus of one subunit to the N-terminus of the second subunit. Like the native CIRV p19, these linked p19 proteins were able to bind to double-stranded siRNAs with nanomolar affinity and discriminate siRNA according to length. In addition, the interdomain linker improved both the stability and binding properties of the p19 dimer. The observed binding properties support the idea that the semi-rigid cross-link favors the folded, binding-competent state of p19. The cross-linked recombinant CIRV-p19s represent novel stabilized suppressors of RNA silencing and may be useful in future biophysical, immunological and cell biology studies.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Steacie Institute for Molecular Sciences
Peer reviewedNo
Identifier10307965
NPARC number12339293
Export citationExport as RIS
Report a correctionReport a correction
Record identifier25ff1df5-4922-4108-b9d8-d62cd47bc3fa
Record created2009-09-11
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)