Solution conformation of asparagine-linked oligosaccharides: alpha(1-2)-, alpha(1-3)-, beta(1-2)-, and beta(1-4)-linked units

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TypeArticle
Journal titleBiochemistry
Volume22
Issue15
Pages36713680; # of pages: 10
Subject1H; ALPHA; analysis; Asparagine; ASPARAGINE-LINKED; ASPARAGINE-LINKED OLIGOSACCHARIDES; CALCULATION; CALCULATIONS; Carbohydrate Conformation; Carbohydrate Sequence; chemical; chemical shift; CHEMICAL-SHIFTS; Comparative Study; conformation; CONFORMATIONAL; ENERGY; energy calculations; ENHANCEMENT; GlcNAc; glycopeptide; Glycopeptides; HIGH-RESOLUTION; Kinetics; LINKAGES; MAGNETIC; Magnetic Resonance Spectroscopy; MAGNETIC-RESONANCE; Models,Molecular; MOLECULE; NMR; NUCLEAR; Nuclear Magnetic Resonance; NUCLEAR-MAGNETIC-RESONANCE; oligosaccharide; Oligosaccharides; OVERHAUSER; OVERHAUSER ENHANCEMENT; PARAMETERS; POTENTIAL; RESIDUES; RESONANCE; shifts; solution; SOLUTION CONFORMATION; Structure-Activity Relationship; Support,Non-U.S.Gov't; UNIT
AbstractThe solution conformation is presented for representatives of each of the major classes of asparaginyl oligosaccharides. In this report the conformation of alpha(1-3)-, alpha(1-2)-, beta(1-2)-, and beta(1-4)-linked units is described. The conformational properties of these glycopeptides were determined by high-resolution 1H nuclear magnetic resonance in conjunction with potential energy calculations. The NMR parameters that were used in this analysis were chemical shifts and nuclear Overhauser enhancements. Potential energy calculations were used to evaluate the preferred conformers available for the different linkages in glycopeptides and to draw conclusions about the behavior in solution of these molecules. It was found that the linkage conformation of the Man alpha 1-3 residues was not affected by substitution either at the 2-position by alpha Man or beta GlcNAc or at the 4-position by beta GlcNAc or by the presence of a bisecting GlcNAc on the adjacent beta Man residue
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberBRISSON1983G
NPARC number9388957
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Record identifier265902d8-134d-4610-87cf-f21a447bdf6f
Record created2009-07-10
Record modified2016-05-09
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