Production of lentiviral vectors encoding recombinant factor VIII expression in serum-free suspension cultures

Download
  1. Get@NRC: Production of lentiviral vectors encoding recombinant factor VIII expression in serum-free suspension cultures (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1590/S1516-89132015060367
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleBrazilian Archives of Biology and Technology
ISSN1516-8913
Volume58
Issue6
Pages923928; # of pages: 6
AbstractLentiviral vector-mediated gene transfer offers several advantages over other gene delivery vectors when considering gene and cell therapy applications. However, using these therapies in clinical applications involves large-scale vector production in an efficient and cost-effective manner. Here we describe a high yield production of a lentivirus encoding recombinant factor VIII in a scalable and GMP-compliant culture system, based on serum free suspension cultures and transient transfection with an inexpensive reagent, polyethylenimine (PEI), reaching a total viral yield of 2.48x108 particles.
Publication date
PublisherBrazilian Archives of Biology and Technology
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); Human Health Therapeutics
Peer reviewedYes
NPARC number21277344
Export citationExport as RIS
Report a correctionReport a correction
Record identifier26e043c9-1916-42c4-9272-2352a3ec2215
Record created2016-03-09
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)