Zinc, a novel structural element found in the family of bacterial adenylate kinases

Download
  1. Get@NRC: Zinc, a novel structural element found in the family of bacterial adenylate kinases (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1021/bi00127a002
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleBiochemistry
ISSN0006-2960
1520-4995
Volume31
Issue12
Pages30383043; # of pages: 6
AbstractThe adk gene from Bacillus stearothermophilus was cloned and overexpressed in Escherichia coli under the control of the lac promoter. The primary structure of B. stearothermophilus adenylate kinase exhibited 76% identity with the enzyme from Bacillus subtilis, 60% identity with the enzyme from Lactococcus lactis, and 42% identity with the enzyme from E. coli. The most striking property of the adenylate kinase from B. stearothermophilus is the presence of a structural zinc atom bound to four cysteines in a zinc finger-like fashion. The ability to coordinate zinc is predicted also for a number of other isoforms of bacterial adenylate kinases. Furthermore, the tightly bound metal ion contributes to the high thermodynamic stability of adenylate kinase from B. stearothermophilus.
Publication date
PublisherAmerican Chemical Society
LanguageEnglish
AffiliationNRC Institute for Biodiagnostics; National Research Council Canada; NRC Institute for Biological Sciences
Peer reviewedYes
NRC number29
NPARC number9148462
Export citationExport as RIS
Report a correctionReport a correction
Record identifier27255db2-03c3-4f41-bb98-8a05aa95b895
Record created2009-06-25
Record modified2016-12-12
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)
Date modified: