Phylogenetic and metabolic diversity of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX)-transforming bacteria in strictly anaerobic mixed cultures enriched on RDX as nitrogen source

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DOIResolve DOI: http://doi.org/10.1016/S0168-6496(03)00216-2
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TypeArticle
Journal titleFEMS Microbiology Ecology
Volume46
Issue2
Pages189196; # of pages: 8
Subjectenv; Nitrogen; Genes
AbstractFive obligate anaerobes that were most closely related to Clostridium bifermentans, Clostridium celerecrescens, Clostridium saccharolyticum, Clostridium butyricum and Desulfovibrio desulfuricans by their 16S rRNA genes sequences were isolated from enrichment cultures using hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) as a nitrogen source. The above isolates transformed RDX at rates of 24.0, 5.4, 6.2, 2.5, 5.5 ?mol h-1 g (dry weight) of cells-1, respectively, to nitrite, formaldehyde, methanol, and nitrous oxide. The present results indicate that clostridia are major strains responsible for RDX removal, and all isolates seemed to mainly transform RDX via its initial reduction to MNX and subsequent denitration. Since clostridia are commonly present in soil, we suggest that they may contribute to the removal of RDX in the subsurface (anoxic) soil.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Peer reviewedNo
NRC number45947
NPARC number3539972
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Record identifier27600fbe-6d5a-4f52-aa18-695f0826aff0
Record created2009-03-01
Record modified2016-05-09
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