Efficient utrophin expression following adenovirus gene transfer in dystrophic muscle

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DOIResolve DOI: http://doi.org/10.1006/bbrc.1997.7936
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TypeArticle
Journal titleBiochemical and Biophysical Research Communications
ISSN0006-291X
Volume242
Issue1
Pages244247; # of pages: 4
Subjectpha; Adenoviridae; Animals; Cytoskeletal Proteins; Gene Therapy; Gene Transfer Techniques; Genetic Vectors; Injections, Intramuscular; Membrane Proteins; Mice; Mice, Inbred mdx; Muscle, Skeletal; Muscular Dystrophy, Animal; Recombinant Proteins; Utrophin
AbstractUtrophin is a homologue of dystrophin, the protein whose absence is responsible for Duchenne muscular dystrophy (DMD). As a first step toward clarifying if adenovirus (AV)-mediated utrophin transfer is a possible option to treat DMD, we have constructed an AV expressing utrophin (AdCMV-Utr) and studied utrophin expression after intramuscular injection of mdx mice, the mouse DMD model. Overexpression of utrophin by AdCMV-Utr was marked and nontoxic. The recombinant utrophin was distributed homogeneously at the surface of the muscle fibers. Its expression was sufficient to restore the normal histochemical pattern of α-sarcoglycan and β-dystroglycan at this site. these two proteins are members of the dystrophin associated protein complex whose distribution is greatly reduced at the surface of the DMD muscle. These data indicate that AV-mediated utrophin transfer is an efficient way of utrophin upregulation in muscle and has the potential of becoming a treatment for DMD.
Publication date
LanguageEnglish
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
NoteUI - 98102816
Peer reviewedNo
Identifier10054637
NRC number41421
NPARC number3539480
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Record identifier27bca0ac-7cf4-4fd1-a01c-1f424c0442f4
Record created2009-03-01
Record modified2016-05-09
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