Impact of wastewater treatment processes on antimicrobial resistance genes and their co-occurrence with virulence genes in Escherichia coli

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DOIResolve DOI: http://doi.org/10.1016/j.watres.2013.11.047
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TypeArticle
Journal titleWater Research
ISSN0043-1354
Volume50
Pages245253; # of pages: 9
SubjectActivated sludge; Antimicrobial resistances; DNA micro-array; Insertion sequences; Physicochemical; Activated sludge process; Bioassay; Biochips; Effluent treatment; Effluents; Genes; Microorganisms; Wastewater treatment; Escherichia coli; aminoglycoside; antibiotic agent; beta lactam antibiotic; DNA; integrase; quinolone; tetracycline derivative; transposase; activated sludge; disinfection; fecal coliform; gene; genome; genotype; public health; sewage treatment; virulence; activated sludge; antibiotic resistance; article; bacterial gene; bacterial genome; bacterial virulence; bacterium isolation; controlled study; DNA microarray; DNA sequence; effluent; Escherichia coli; gene insertion; genotype; genotyping technique; influent; nonhuman; physical chemistry; priority journal; waste water management; waste water treatment plant; water pollutant
AbstractAn increase in the frequency of antimicrobial resistance genes (ARGs) in bacteria including Escherichia coli could be a threat to public health. This study investigated the impact of activated sludge and physicochemical wastewater treatment processes on the prevalence of ARGs in E. coli isolates. In total, 719 E. coli were isolated from the influent and effluent (prior to disinfection) of two activated sludge and two physicochemical municipal treatment plants, and genotyped using DNA microarrays. Changes in the abundance of ARGs in the E. coli population were different for the two treatment processes. Activated sludge treatment did not change the prevalence of ARG-possessing E. coli but increased the abundance of ARGs in the E. coli genome while physicochemical treatment reduced both the prevalence of ARG-carrying E. coli as well as the frequency of ARGs in the E. coli genome. Most E. coli isolates from the four treatment plants possessed ARGs of multiple antimicrobial classes, mainly aminoglycoside, β-lactams, quinolone and tetracyclines. In addition these isolates harboured DNA insertion sequence elements including integrase and transposase. A significant positive association was found between the occurrence of ARGs and virulence genotypes. © 2013 Elsevier Ltd.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC)
Peer reviewedYes
NPARC number21270830
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Record identifier29cadd30-4e40-443b-977a-99280f37691c
Record created2014-02-17
Record modified2016-05-09
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