Comparative genomics profiling of clinical isolates of Aeromonas salmonicida using DNA microarrays

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DOIResolve DOI: http://doi.org/10.1186/1471-2164-7-43
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TypeArticle
Journal titleBMC Genomics
ISSN14712164
Volume7
Pages43:143:15
Subjectclassification; genetics; isolation and purification; pathogenicity; microbiology; methods; Aeromonas salmonicida
AbstractBackground: Aeromonas salmonicida has been isolated from numerous fish species and shows wide variation in virulence and pathogenicity. As part of a larger research program to identify virulence genes and candidates for vaccine development, a DNA microarray was constructed using a subset of 2024 genes from the draft genome sequence of A. salmonicida subsp. salmonicida strain A449. The microarray included genes encoding known virulence-associated factors in A. salmonicida and homologs of virulence genes of other pathogens. We used microarray-based comparative genomic hybridizations (M-CGH) to compare selected A. salmonicida sub-species and other Aeromonas species from different hosts and geographic locations. Results: Results showed variable carriage of virulence-associated genes and generally increased variation in gene content across sub-species and species boundaries. The greatest variation was observed among genes associated with plasmids and transposons. There was little correlation between geographic region and degree of variation for all isolates tested. Conclusion: We have used the M-CGH technique to identify subsets of conserved genes from amongst this set of A. salmonicida virulence genes for further investigation as potential vaccine candidates. Unlike other bacterial characterization methods that use a small number of gene or DNA-based functions, M-CGH examines thousands of genes and/or whole genomes and thus is a more comprehensive analytical tool for veterinary or even human health research.
Publication date
PublisherBioMed Central Ltd.
Copyright notice© 2006 Nash et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
LanguageEnglish
AffiliationNRC Institute for Marine Biosciences; National Research Council Canada; NRC Institute for Biological Sciences
Peer reviewedYes
NRC number55835
1581
NPARC number3538350
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Record identifier2a41a6dc-624d-4a67-904b-a40d12adeb3f
Record created2009-03-01
Record modified2016-05-09
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