Localization and speciation of selenium and mercury in Brassica juncea: implications for Se–Hg antagonism

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DOIResolve DOI: http://doi.org/10.1039/b514954a
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TypeArticle
Journal titleJournal of Analytical Atomic Spectrometry
ISSN0267-9477
1364-5544
Volume21
Issue4
Pages404
AbstractThe occurrence and form of selenium and mercury were investigated in Indian Mustard, Brassica juncea, a selenium accumulating plant, which had been co-exposed to varying concentration levels of these two elements. Plants were grown and exposed in hydroponic solutions. Following exposure, root exudates were collected in fresh solutions and the head-space around the aerial portions of the plants was sampled. These samples and the harvested plant tissues were then processed for determination of Se and Hg-containing compounds. For the plant tissues, roots, stems and leaves were separated and extracted using a sequential procedure that removed water-soluble species, water-soluble proteins, and dodecyl sulfate-soluble proteins. Size exclusion chromatography allowed further fractionation. High molecular-weight selenium/mercury-containing compounds were found primarily in the plant root extract. Evidence suggests that a Se and Hg complex of high molecular weight may be protein associated. For the analysis of exudate solutions, ion-pairing reversed phase chromatography coupled to ICP-MS was used. Multiple selenium and mercury species were detected, with one mercury-containing compound observed eluting near selenocystine. Plant head-space was sampled with solid phase microextraction and analyzed with GC-ICP-MS and GC-TOFMS. Apart from the primary selenium volatiles and elemental mercury, no volatile species simultaneously containing Se and Hg could be detected.
Publication date
PublisherRoyal Society of Chemistry
LanguageEnglish
AffiliationNational Research Council Canada
Peer reviewedYes
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NPARC number23001832
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Record identifier2aa602b4-407c-4bc9-a6ee-0659cb6cd41c
Record created2017-04-13
Record modified2017-04-13
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