Immunofluorescence imaging as a tool for studying the pharmacokinetics of a human monoclonal single chain fragment antibody

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DOIResolve DOI: http://doi.org/10.1109/TMI.2002.806275
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TypeArticle
Journal titleIEEE Transactions on Medical Imaging
ISSN0278-0062
1558-254X
Volume21
Issue10
Pages13171323; # of pages: 7
AbstractWe have used immunofluorescence imaging to study binding of a monoclonal antibody fragment to subcutaneously implanted human melanoma cells in nude mice. The data acquired using this nontraditional approach was then analyzed using standard pharmacokinetic methods to produce estimates of k/sub e/ (0.06 h/sup -1/), t/sub 1/2/ (16 h), mean residency time (23.4 h) and percent exposure of the antibody to the tumor (40%). To our knowledge this is the first time standard pharmacokinetic analyzes have been conducted on immunofluorescence imaging data. The combination of this novel imaging technique and standard pharmacokinetic analytical methods should prove to be a useful tool for comparing the properties of antibody fragments in animal models.
Publication date
PublisherIEEE
AffiliationNRC Institute for Biodiagnostics; National Research Council Canada
Peer reviewedNo
NRC number1969
NPARC number9147671
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Record identifier2c2dbcc6-10f0-4197-b081-afbcb91370ee
Record created2009-06-25
Record modified2016-09-12
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