Preparation and characterization of bacterial protein complexes for structural analysis

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DOIResolve DOI: http://doi.org/10.1016/S1876-1623(08)76001-2
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TypeBook Chapter
Book titleAdvances in Protein Chemistry and Structural Biology
ISBN978-0-12-374442-5
Volume76
Pages142; # of pages: 42
AbstractBacteria mediate a large variety of biological processes using protein complexes. These complexes range from simple binary heterodimeric enzymes to more complex multi-subunit complexes that can be described as macromolecular machines. A key to understanding how these complexes function is obtaining structural information using methods that include electron microscopy, small-angle X-ray scattering, NMR spectroscopy, and X-ray crystallography. Here we describe a variety of approaches to the expression, purification, and biophysical characterization of bacterial protein complexes as a prerequisite to structural analysis. We also give several examples of the kinds of information these different biophysical approaches can provide and various experimental approaches to obtaining structure information for a given system. Further, we describe several examples of protein complexes where we have obtained structural data that have led to new biological insights.
Publication date
LanguageEnglish
AffiliationHuman Health Therapeutics; NRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedYes
NRC number50671
NPARC number19978841
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Record identifier2cb38168-105e-4e32-8699-c407e6a4f4e7
Record created2012-05-23
Record modified2016-05-09
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