Imaging nanometer domains of β-adrenergic receptor complexes on the surface of cardiac myocytes

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DOIResolve DOI: http://doi.org/10.1038/nchembio726
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TypeArticle
Journal titleNature Chemical Biology
ISSN1552-4450
Volume1
Issue4
Pages196202; # of pages: 7
SubjectDomain; receptor; complexes; complex; surface
AbstractThe contraction of cardiac myocytes is initiated by ligand binding to adrenergic receptors¹,² contained in nanoscale multiprotein complexes called signalosomes³. The composition and number of functional signalosomes within cardiac myocytes defines the molecular basis of the response to adrenergic stimuli³⁻⁶. For the first time, we demonstrated the ability of near-field scanning optical microscopy to visualize β-adrenergic receptors at the nanoscale in situ. On H9C2 cells, mouse neonatal and mouse embryonic cardiac myocytes, we showed that functional receptors are organized into multiprotein domains of ~140 nm average diameter. Colocalization experiments in primary cells at the nanometer scale showed that 15?20% of receptors were preassociated in caveolae. These nanoscale complexes were sufficient to effect changes in ligand-induced contraction rate without the requirement for substantial changes in receptor distribution in the cellular membrane. Using fluorescence intensities associated with these nanodomains, we estimated the receptor density within the observed nanometer features and established a lower limit for the number of receptors in the signalosome.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Steacie Institute for Molecular Sciences; NRC Institute for Biological Sciences
Peer reviewedNo
Identifier20466353
NRC numberIANOUL2005
NPARC number12333646
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Record identifier2cf26470-9dda-4389-a98f-8bda4bdea6b9
Record created2009-09-10
Record modified2016-05-09
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