Expression of single-domain antibodies in bacterial systems

  1. Get@NRC: Expression of single-domain antibodies in bacterial systems (Opens in a new window)
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Journal titleMethods in Molecular Biology
Pages257275; # of pages: 19
Subjectnanobody; recombinant antibody; hybrid protein; antibody library; antibody production; article; bacterium; culture medium; cytoplasm; Escherichia coli; expression vector; gene expression system; heavy chain; molecular cloning; nonhuman; phage display; priority journal; procedures; protein domain; protein expression; protein isolation; protein purification; reproducibility; antibody affinity; gene expression; gene library; gene vector; genetics; immunology; isolation and purification; metabolism; Antibody Affinity; Bacteria; Cloning, Molecular; Escherichia coli; Gene Expression; Gene Library; Genetic Vectors; Recombinant Fusion Proteins; Single-Domain Antibodies
AbstractIn this chapter we describe in detail the current protocols that are used to express single-domain antibodies in bacteria. Bacteria are among the most common expression systems for expressing recombinant proteins. We present different approaches for carrying out periplasmic and cytoplasmic expression, as well as small-scale and large-scale expression. In addition, we discuss the advantages and possible drawbacks of each protocol. We present data related to expression vectors, expression conditions, methods of protein extraction and purification, and yield and purity analysis of sdAbs. We also highlight important points that need to be considered before sdAbs that have been expressed in bacteria are used either in vitro or in vivo. © 2012 Springer Science+Business Media, LLC.
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AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Biological Sciences (IBS-ISB)
Peer reviewedYes
NPARC number21269464
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Record identifier2e30ee19-a743-4733-8516-a36d13203a8c
Record created2013-12-12
Record modified2016-05-09
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