Structural And Biological Characterization of a Capsular Polysaccharide Produced by Staphylococcus haemolyticus: J.Bacteriol.

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TypeArticle
Journal titleJ Bacteriol.
SubjectACID; Antibodies; antibody; assay; Bacteria; Canada; capsular polysaccharide; capsule; DNA; electron; GENE; Genes; Genome; Human; Humans; In Vitro; INFECTION; Laboratories; Microbiology; Microscopy; MUTANT; PHASE; POLYSACCHARIDE; Polysaccharides; REGION; REPEATING UNIT; SEQUENCE; STRAIN; STRAINS; STRUCTURAL; STRUCTURAL CHARACTERIZATION; structure; SUBSTITUENT; sugar; transferase; Transferases; TRISACCHARIDE; UNIT
AbstractThe DNA sequence of the genome of Staphylococcus haemolyticus JCSC1435 revealed a putative capsule operon composed of 13 genes in tandem. The first seven genes (capShABCDEFG) showed >/=57% similarity with the S. aureus cap5(8) locus. However, the capShHIJKLM genes are unique to S. haemolyticus and include genes encoding a putative flippase, an aminotransferase, two glycosyl transferases, and a transcriptional regulator. Capsule-like material was readily apparent by immmunoelectron microscopy on bacteria harvested in the post-exponential phase of growth. Electron micrographs of a JCSC1435 mutant with a deleted cap region lacked the capsule-like material. Both strains produced small amounts of surface-associated material that reacted with antibodies to poly-glutamic acid. S. haemolyticus cap genes were amplified from four of seven clinical isolates of S. haemolyticus from humans, and three of these strains produced a serologically cross-reactive capsular polysaccharide. In vitro assays showed that the acapsular mutant strain showed greater biofilm formation but was more susceptible to complement-mediated opsonophagocytic killing compared to the parent strain. Structural characterization of capsule purified from S. haemolyticus strain JCSC1435 showed a trisaccharide repeating unit: -3-alpha-L-FucNAc-3-(2-NAc-4-N-Asp-2,4,6-trideoxy-beta-D-Glc)-4-alpha-D-Gl cNAc-. This structure is unique among staphylococcal polysaccharides in that its composition includes a trideoxy sugar residue with aspartic acid as an N-acyl substituent
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberFLAHAUT2007
NPARC number9360670
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Record identifier2e525cd7-b79d-4ed9-b750-2a6d90081875
Record created2009-07-10
Record modified2016-05-09
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