Interaction of saponin 1688 with phase separated lipid bilayers

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DOIResolve DOI: http://doi.org/10.1016/j.bbamem.2017.03.024
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TypeArticle
Journal titleBiochimica et Biophysica Acta (BBA) - Biomembranes
BBA Biomembranes
ISSN0005-2736
Volume1859
Issue7
Pages12631272
Subjectsaponin; lipid bilayer; atomic force microscopy; fluorescence; dye leakage
AbstractSaponins are a diverse family of naturally occurring plant triterpene or steroid glycosides that have a wide range of biological activities. They have been shown to permeabilize membranes and in some cases membrane disruption has been hypothesized to involve saponin/cholesterol complexes. We have examined the interaction of steroidal saponin 1688-1 with lipid membranes that contain cholesterol and have a mixture of liquid-ordered (Lo) and liquid-disordered (Ld) phases as a model for lipid rafts in cellular membranes. A combination of atomic force microscopy (AFM) and fluorescence was used to probe the effect of saponin on the bilayer. The results demonstrate that saponin forms defects in the membrane and also leads to formation of small aggregates on the membrane surface. Although most of the membrane damage occurs in the liquid-disordered phase, fluorescence results demonstrate that saponin localizes in both ordered and disordered membrane phases, with a modest preference for the disordered regions. Similar effects are observed for both direct incorporation of saponin in the lipid mixture used to make vesicles/bilayers and for incubation of saponin with preformed bilayers. The results suggest that the initial sites of interaction are at the interface between the domains and surrounding disordered phase. The preference for saponin localization in the disordered phase may reflect the ease of penetration of saponin into a less ordered membrane, rather than the actual cholesterol concentration in the membrane. Dye leakage assays indicate that a high concentration of saponin is required for membrane permeabilization consistent with the supported lipid bilayer experiments.
Publication date
PublisherElsevier
LanguageEnglish
AffiliationMeasurement Science and Standards; Aquatic and Crop Resource Development; National Research Council Canada
Peer reviewedYes
NRC numberNRC-ACRD-56338
NPARC number23001930
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Record identifier2eda06a4-50d2-4f90-bdc5-8a5e1baed39c
Record created2017-06-02
Record modified2017-06-02
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