Structure and immunogenicity of the rough-type lipopolysaccharide from the periodontal pathogen Tannerella forsythia

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DOIResolve DOI: http://doi.org/10.1128/CVI.00139-13
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TypeArticle
Journal titleClinical and Vaccine Immunology
ISSN1556-6811
Volume20
Issue6
Pages920930; # of pages: 11
Subjectbacterium lipopolysaccharide; galactose; glucosamine; interleukin 1; interleukin 6; mannose; phosphate; phosphoethanolamine; tumor necrosis factor alpha; article; carbohydrate analysis; controlled study; cytokine production; fetal calf serum; host pathogen interaction; human; human cell; immunogenicity; immunostimulation; macrophage activation; nonhuman; periodontium; priority journal; structure analysis; surface property; Tannerella forsythensis
AbstractTannerella forsythia is a Gram-negative anaerobic organism that inhabits subgingival plaque biofilms and is covered with a so far unique surface layer composed of two glycoproteins. It belongs to the so-called "red complex" of bacteria comprising species that are associated with periodontal disease. While the surface layer glycoprotein glycan structure had been elucidated recently and found to be a virulence factor, no structural data on the lipopolysaccharide (LPS) of this organism were available. In this study, the T. forsythia LPS structure was partially elucidated by a combined mass spectrometry (MS) and nuclear magnetic resonance spectroscopy (NMR) approach and initial experiments to characterize its immunostimulatory potential were performed. The T. forsythia LPS is a complex, rough-type LPS with a core region composed of one 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) residue, three mannose residues, and two glucosamine residues. MS analyses of O-deacylated LPS proved that, in addition, one phosphoethanolamine residue and most likely one galactose-phosphate residue were present, however, their positions could not be identified. Stimulation of human macrophages with T. forsythia LPS resulted in the production of the proinflammatory cytokines interleukin-1 (IL-1), IL-6, and tumor necrosis factor alpha in a dose-dependent manner. The response to T. forsythia LPS was observed only upon stimulation in the presence of fetal calf serum (FCS), whereas no cytokine production was observed in the absence of FCS. This finding suggests that the presence of certain additional cofactors is crucial for the immune response induced by T. forsythia LPS. Copyright © 2013, American Society for Microbiology. All Rights Reserved.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Biological Sciences (IBS-ISB)
Peer reviewedYes
NPARC number21269604
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Record identifier2fa3ed54-2681-4fb8-aab5-c9f80f479b37
Record created2013-12-13
Record modified2016-05-09
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