FITC delivery into plant cells using magnetic single-walled carbon nanotubes

Download
  1. Get@NRC: FITC delivery into plant cells using magnetic single-walled carbon nanotubes (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1166/jnn.2012.6449
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleJournal of Nanoscience and Nanotechnology
ISSN1533-4880
Volume12
Issue8
Pages62876293; # of pages: 7
SubjectCovalently bonded; Endosomes; FITC Delivery; Fluorescein isothiocyanate; Magnetic force; Magnetic Single-Walled Carbon Nanotubes (SWCNTs); Nuclear membranes; Plant cells; Protoplast of Canola and Carrot; TEM images; Cell membranes; Plant cell culture; Single-walled carbon nanotubes (SWCN)
AbstractIn this paper, fluoresce in isothiocyanate (FITC) was covalently bonded with magnetic single-walled carbon nanotubes (mSWCNTs) that were purified using our previous method. To demonstrate our design, mSWCNT-FITC was delivered into plant cells (canola and carrot cells) driven by external magnetic forces. From FACS results, the FITC delivery efficiency was about 100% for both two canola and carrot protoplasts, which were further confirmed by the confocal and sectional TEM images. Some mSWCNTs were found trapped both inside the endosomes of canola protoplast and outside endosome near the nuclear membrane of carrot protoplast according to the sectional TEM images. All results showed that mSWCNT is a good delivery carrier for biomolecules.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); Security and Disruptive Technologies
Peer reviewedYes
NPARC number21270230
Export citationExport as RIS
Report a correctionReport a correction
Record identifier2fc29e6c-1675-4e3c-8ad5-9751c6419f2d
Record created2014-01-14
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)