Mytilus edulis adhesive protein (MAP) as an enzyme immobilization matrix in the fabrication of enzyme-based electrodes

Download
  1. Get@NRC: Mytilus edulis adhesive protein (MAP) as an enzyme immobilization matrix in the fabrication of enzyme-based electrodes (Opens in a new window)
AuthorSearch for: ; Search for:
TypeArticle
Journal titleElectroanalysis
Volume10
Issue17
Pages11931199; # of pages: 7
SubjectBiosensor; Electrochemical; Bioadhesive; Immobilization; Glucose oxidase
AbstractA simple enzyme immobilization technique using an adhesive protein isolated from Mytilus edulis blue mussels was optimized for constructing a glucose oxidase (GOD) based electrode. Owing to the presence of 10–15 % of 3,4-dihydroxyphenylalanine residues, the mussel adhesive protein (MAP) was easily oxidized to form a stable protein film on platinum, gold and glassy carbon electrodes. Covalent attachment of glucose oxidase to adhesive protein modified electrodes was attained since the oxidized form of the L-Dopa moieties was very reactive towards various compounds containing amino, alcohol and thiol groups. Glucose detection was performed using MAP/GOD modified electrodes at +0.8 V in 0.1 M phosphate buffer, pH 5. Platinum modified electrodes (Pt/MAP/GOD) exhibited a considerably higher sensitivity (1.28 μA/mM) in comparison to its gold (Au/MAP/GOD, 2.92 nA/mM) or glassy carbon (GC/MAP/GOD, 2.62 nA/mM) counterparts. Tetrachloro-1,4-benzoquinone (TCBQ), an oxidation product of pentachlorophenol, was detected using MAP/GOD modified electrodes at +0.45 V in a deaerated 0.1 M tartaric acid buffer, pH 3.5 containing 40 mM glucose. In steady state, the current response of the Au/MAP/GOD electrode was higher (6.62 nA nM−1cm−2, t95 = 83 s, 5 nM) than the GC/MAP/GOD electrode (3.78 nA nM−1 cm−2, 8 nM). In flow injection analysis, GC/MAP/GOD electrodes exhibited a linear response for TCBQ ranging from 10 nM to 1 μM (3.96 nAs nM−1) with a detection limit of 10 nM. The modified glassy carbon enzyme based electrode retained 90 % and 87 % of its activity after 150 and 250 repeated injections.
Publication date
AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Peer reviewedNo
NRC number41796
NPARC number3538593
Export citationExport as RIS
Report a correctionReport a correction
Record identifier2fdd8975-6df3-4bbc-8bcb-53506c041783
Record created2009-03-01
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)