Use of Fourier-transform infrared spectroscopy to quantify immunoglobulin G concentration and an analysis of the effect of signalment on levels in canine serum

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DOIResolve DOI: http://doi.org/10.1016/j.vetimm.2014.10.011
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TypeArticle
Journal titleVeterinary Immunology and Immunopathology
ISSN0165-2427
Volume163
Issue1-Feb
Pages815; # of pages: 8
Subjectimmunoglobulin G; analysis of variance; calibration; correlation coefficient; dog; female; health status; immunodiffusion; infrared spectroscopy; male; partial least squares regression; prediction; radial immunodiffusion; serum; Animalia; Canis familiaris
AbstractDeficiency in immunoglobulin G (IgG) is associated with an increased susceptibility to infections in humans and animals, and changes in IgG levels occur in many disease states. In companion animals, failure of transfer of passive immunity is uncommonly diagnosed but mortality rates in puppies are high and more than 30% of these deaths are secondary to septicemia. Currently, radial immunodiffusion (RID) and enzyme-linked immunosorbent assays are the most commonly used methods for quantitative measurement of IgG in dogs. In this study, a Fourier-transform infrared spectroscopy (FTIR) assay for canine serum IgG was developed and compared to the RID assay as the reference standard. Basic signalment data and health status of the dogs were also analyzed to determine if they correlated with serum IgG concentrations based on RID results.Serum samples were collected from 207 dogs during routine hematological evaluation, and IgG concentrations determined by RID. The FTIR assay was developed using partial least squares regression analysis and its performance evaluated using RID assay as the reference test. The concordance correlation coefficient was 0.91 for the calibration model data set and 0.85 for the prediction set. A Bland-Altman plot showed a mean difference of -89. mg/dL and no systematic bias. The modified mean coefficient of variation (CV) for RID was 6.67%, and for FTIR was 18.76%.The mean serum IgG concentration using RID was 1943. ±. 880. mg/dL based on the 193 dogs with complete signalment and health data. When age class, gender, breed size and disease status were analyzed by multivariable ANOVA, dogs <2 years of age (. p=. 0.0004) and those classified as diseased (. p=. 0.03) were found to have significantly lower IgG concentrations than older and healthy dogs, respectively.
Publication date
PublisherElsevier
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC)
Peer reviewedYes
NPARC number21275693
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Record identifier3193815a-9fd0-4b91-90ae-56b4fbd1c3f6
Record created2015-07-14
Record modified2016-05-09
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