Exploring the cell uptake mechanism of phospholipid and polyethylene glycol coated gold nanoparticles

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DOIResolve DOI: http://doi.org/10.1088/0957-4484/23/4/045103
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TypeArticle
Journal titleNanotechnology
ISSN0957-4484
Volume23
Issue4
Article number45103
SubjectBiodistributions; Biomedical applications; Cell uptake; In-vivo; Nanoparticle sizes; SEM and TEM; Sodium salt; Structural similarity; Biocompatibility; Cell membranes; Thermoplastics; Polyethylene glycols; 1 palmitoyl 2 oleoylglycero 3 phosphoglycerol; 1-palmitoyl-2-oleoylglycero-3-phosphoglycerol; macrogol derivative; metal nanoparticles; phosphatidylglycerol; cell death; drug effect; endocytosis; tumor cell line; ultrastructure; Phosphatidylglycerols; Polyethylene Glycols
AbstractRecently, there has been a lot of interest in using gold nanoparticles (GNPs) for biomedical applications due to their biocompatibility. To increase GNP cell uptake and circulation half-life, and to improve its bio-distribution invivo, we chose to coat GNPs with 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1- rac-glycerol) (sodium salt) (POPG) and polyethylene glycol (PEG). Two different methods were used to synthesize POPG-GNPs or PEG-GNPs, but the resulting nanoparticle sizes and morphologies were similar. Under the same incubation conditions, POPG-GNPs can be uptaken quicker than PEG-GNPs by cellsspecifically, the maximum uptake was 8h versus 16h after incubation. In addition, the uptake amount of POPG-GNPs was more than that of PEG-GNPs. The uptake processes were confirmed by SEM and TEM images. The main reason for the greater uptake of POPG-GNPs can be attributed to the structural similarities between the POPG coating and the cell membrane as well as GNP aggregation.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); National Institute for Nanotechnology
Peer reviewedYes
NPARC number21270274
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Record identifier338e9260-03b5-4873-8840-694312fb157e
Record created2014-01-20
Record modified2016-05-09
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