Mutations conferring resistance to azide in Escherichia coli occur primarily in the secA gene

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TypeArticle
Journal titleJournal of Bacteriology
Volume172
Issue11
Pages66076610; # of pages: 4
AbstractMutant strains of Escherichia coli were screened for the ability to grow on L agar plates containing 3.4 or 4.6 mM sodium azide. Most mutants had mutations located in the leucine region, presumably at the azi locus. Two of these mutants were found to have a mutation in the secA gene, but expression of the resistance phenotype also required the presence of upstream gene X. While a plasmid carrying the X-secA mutant gene pair was able to confer azide resistance to a sensitive host, a similar plasmid harboring the wild-type secA allele rendered a resistant strain sensitive to azide, indicating codominance of the two alleles. That azide inhibits SecA is consistent with the fact that SecA has ATPase activity, an activity that is often prone to inhibition by azide.
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LanguageEnglish
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NPARC number12328442
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Record identifier3397a7a0-6ab2-44a7-99f1-ed62f45cc590
Record created2009-09-10
Record modified2016-05-09
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