Determination of selenomethionine in yeast using CNBr derivatization and species specific isotope dilution GC ICP-MS and GC-MS

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DOIResolve DOI: http://doi.org/10.1039/b410543e
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TypeArticle
Journal titleJournal of Analytical Atomic Spectrometry
ISSN0267-9477
Volume19
Issue11
Pages14481453; # of pages: 6
AbstractA comparison of several published and in-house methods for quantitation of selenomethionine (SeMet) in yeast was undertaken using species specific isotope dilution (ID) with a 74Se enriched SeMet. An in-house method was based on digestion of samples by refluxing for 16 h with 4 M methanesulfonic acid, derivatization of SeMet with cyanogen bromide and extraction into chloroform for determination by GC ICP-MS. Concentrations of 3434 ± 19 and 3419 ± 15 μg g−1 (one standard deviation, n = 6) with relative standard deviations of 0.55% and 0.42% for SeMet were obtained in yeast based on measurement of 78Se/74Se and 82Se/74Se ratios, respectively, in agreement with a value of 3417 ± 27 μg g−1 (one standard deviation, n = 6) obtained using ID GC-MS detection based on the ratio of 106/100 of SeCN+ ion. The SeMet accounts for 67% of the total Se in the sample. A method detection limit (three standard deviations) of 0.9 μg g−1 was estimated for SeMet based on a 0.25 g subsample. Significantly lower concentrations of 2220 ± 7 and 2215 ± 9 μg g−1 (one standard deviation, n = 4) with RSDs of 0.30 and 0.41% were obtained by ID GC ICP-MS using 78Se/74Se and 82Se/74Se ratios, respectively, following digestion with 2% SnCl2 in 0.1 M HCl and reaction with CNBr to form volatile CH3SeCN. Similar SeMet concentrations of 3415 ± 200 and 3447 ± 198 μg g−1 (one standard deviation, n = 6) and significantly degraded precisions of 5.86 and 5.74%, respectively, were obtained in yeast using 78Se/74Se and 82Se/74Se ratios, respectively, following digestion with 4 M methanesulfonic acid and derivatization with methyl chloroformate.
Publication date
LanguageEnglish
AffiliationNRC Institute for National Measurement Standards; National Research Council Canada
Peer reviewedNo
Identifier10391186
NRC number455
NPARC number8897067
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Record identifier34226b04-7372-41e3-898d-737948d55477
Record created2009-04-22
Record modified2016-05-09
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