Global small-angle X-ray scattering data analysis for multilamellar vesicles: the evolution of the scattering density profile model

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DOIResolve DOI: http://doi.org/10.1107/S1600576713029798
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TypeArticle
Journal titleJournal of Applied Crystallography
ISSN0021-8898
Volume47
Issue1
Pages173180; # of pages: 8
SubjectExperimental errors; Multi-lamellar vesicles; Scattering density; Small angle X-ray scattering; Small-angle x-rays; Structural information; Structural parameter; Unilamellar vesicle; Binary mixtures; Crystalline materials; Deuterium; Genetic algorithms; Scattering; X ray scattering; X rays
AbstractThe highly successful scattering density profile (SDP) model, used to jointly analyze small-angle X-ray and neutron scattering data from unilamellar vesicles, has been adapted for use with data from fully hydrated, liquid crystalline multilamellar vesicles (MLVs). Using a genetic algorithm, this new method is capable of providing high-resolution structural information, as well as determining bilayer elastic bending fluctuations from standalone X-ray data. Structural parameters such as bilayer thickness and area per lipid were determined for a series of saturated and unsaturated lipids, as well as binary mixtures with cholesterol. The results are in good agreement with previously reported SDP data, which used both neutron and X-ray data. The inclusion of deuterated and non-deuterated MLV neutron data in the analysis improved the lipid backbone information but did not improve, within experimental error, the structural data regarding bilayer thickness and area per lipid. © 2014 International Union of Crystallography.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Canadian Neutron Beam Centre (CNBC-CCFN)
Peer reviewedYes
NPARC number21272205
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Record identifier353cf9f6-14f1-42c5-a8c6-8d8186d79347
Record created2014-07-23
Record modified2016-05-09
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