Rapid detection of aneuploidy following the generation of mutants in Candida albicans

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DOIResolve DOI: http://doi.org/10.1007/978-1-61779-539-8_3
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TypeBook Chapter
Book titleHost-Fungus Interactions : Methods and Protocols
Series titleMethods in Molecular Biology
ISSN1064-3745
ISBN978-1-61779-538-1
Volume845
Pages4149; # of pages: 9
AbstractTechniques used to generate mutants in Candida albicans commonly result in additional and undesired genetic rearrangements. Detection of aneuploidy is, therefore, an important step forward in the quality control of mutant phenotypes. In this chapter, we describe how to extract genomic DNA and perform a quantitative multiplex PCR to compare the karyotype of any mutant strain to that of its parent and allow the detection of any unwanted aneuploidy.
Publication date
PublisherSpringer
LanguageEnglish
AffiliationNational Research Council Canada
Peer reviewedYes
NRC publication
This is a non-NRC publication

"Non-NRC publications" are publications authored by NRC employees prior to their employment by NRC.

NRC number53172
NPARC number21268235
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Record identifier354b3b07-d7a8-4847-9675-9222c3a2a444
Record created2013-06-07
Record modified2016-05-09
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