Label-Free Reading of Microarray-Based Immunoassays with Surface Plasmon Resonance Imaging

Download
  1. Get@NRC: Label-Free Reading of Microarray-Based Immunoassays with Surface Plasmon Resonance Imaging (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1021/ac049318qS0003-2700(04)09318-7
AuthorSearch for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleAnalytical Chemistry
ISSN00032700
Volume76
Issue24
Pages72577262; # of pages: 6
AbstractA simple method is presented for patterning of protein antigens at a gold surface for use in surface plasmon resonance (SPR) imaging experiments. Microfluidic devices fabricated from poly(dimethylsiloxane) were used to flow various fluids over a gold substrate in spatially defined channels. This technique was used to pattern the surface chemistry of the gold as well as to adsorb antigens from solution to the modified substrates. The resulting antigen arrays were probed with complementary antibodies in order to demonstrate the effectiveness of the patterning for antibody capture experiments. SPR imaging was used to aid in the optimization of array fabrication and to observe the interactions of unlabeled antibodies with these microarrays. This work presents a means of fabricating microarrays with controlled surface density of antigens. SPR imaging provides both quantitative and qualitative evaluation of antibody binding in a label free format.
Publication date
AffiliationNational Research Council Canada; National Institute for Nanotechnology
Peer reviewedNo
Identifier10308271
NPARC number12327947
Export citationExport as RIS
Report a correctionReport a correction
Record identifier354e3038-d9c8-4e5a-ad27-1f24fe533db2
Record created2009-09-10
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)