Effect of Freezing on the Biaxial Mechanical Properties of Arterial Samples

Download
  1. (PDF, 1 MB)
  2. Get@NRC: Effect of Freezing on the Biaxial Mechanical Properties of Arterial Samples (Opens in a new window)
DOIResolve DOI: http://doi.org/10.4236/jbise.2010.37088
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleJournal of Biomedical Science and Engineering
Volume3
Issue7
Pages645652; # of pages: 8
SubjectThoracic Aorta; Mechanical Testing; Physiological Solution; Cryopreservation; Cryoprotective Agent
AbstractLittle mechanical data is available on human arteries because of the difficulty of testing artery samples often obtained from autopsy, while arteries are still considered “fresh”. Various solutions mimicking the physiological environment have been used to preserve artery samples from harvesting to testing. Cryopreservation might provide a means to preserve the mechanical properties of arteries for days or weeks after harvesting. The objective of this study is to investigate the effect of several preservation methods, including simplified cryopreservation methods, on the passive mechanical properties of arteries. Eighteen fresh cruciform samples were mechanically tested. Samples were divided in three groups based on preservation medium and freezing method: isotonic saline solution, Krebs-Henseleit buffer solution with dimethyl sulfoxide (DMSO), and dipped in liquid nitrogen. In each group, half of the samples were stored at -20? and the other half at -80?. Two months later, all the tissues were thawed at 4? and mechanical tests were repeated. Preservation of arteries for two months in Krebs solution with DMSO (at -20? or at -80?) or in isotonic saline solution at -20? were the methods that least changed the mechanical properties of the arteries.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Industrial Materials Institute
Peer reviewedYes
NRC number53667
NPARC number15990716
Export citationExport as RIS
Report a correctionReport a correction
Record identifier36e4c626-092a-453f-a7a5-afd89d190da4
Record created2010-11-02
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)