Relating centromeric topography in fixed human chromosomes to -satellite DNA and CENP-B distribution

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DOIResolve DOI: http://doi.org/10.1159/000348744
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TypeArticle
Journal titleCytogenetic and Genome Research
ISSN1424-8581
Volume139
Issue4
Pages234242; # of pages: 9
Subjectcentromere protein B; satellite DNA; article; atomic force microscopy; centromere; comparative study; controlled study; DNA sequence; fluorescence microscopy; fluorescent lighting; human; human cell; human chromosome; metaphase chromosome; priority journal; protein localization; Cell Line, Tumor; Centromere; Centromere Protein B; Chromatin; Chromosomes, Human, Pair 17; DNA Probes; DNA, Satellite; Fluorescent Antibody Technique; Humans; In Situ Hybridization, Fluorescence; Lymphocytes; Metaphase; Microscopy, Atomic Force; Tissue Fixation
AbstractDespite extensive analyses on the centromere and its associated proteins, detailed studies of centromeric DNA structure have provided limited information about its topography in condensed chromatin. We have developed a method with correlative fluorescence light microscopy and atomic force microscopy that investigates the physical and structural organization of -satellite DNA sequences in the context of its associated protein, CENP-B, on human metaphase chromosome topography. Comparison of centromeric DNA and protein distribution patterns in fixed homologous chromosomes indicates that CENP-B and -satellite DNA are distributed distinctly from one another and relative to observed centromeric ridge topography. Our approach facilitates correlated studies of multiple chromatin components comprising higher-order structures of human metaphase chromosomes. © 2013 S. Karger AG, Basel.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Steacie Institute for Molecular Sciences (SIMS-ISSM)
Peer reviewedYes
NPARC number21269955
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Record identifier36ff577c-e59c-4f70-b977-cbfca7751a4a
Record created2013-12-13
Record modified2016-05-09
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