One-step purification of palmatine and its derivative dl-tetrahydropalmatine from Enantia chlorantha using high-performance displacement chromatography

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DOIResolve DOI: http://doi.org/10.1016/j.chroma.2008.08.112
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TypeArticle
Journal titleJournal of Chromatography A
ISSN0021-9673
Volume1208
Issue1-2
Pages4753; # of pages: 7
Subjecthigh-performance displacement chromatography; HPDC; palmatine; dl-Tetrahydropalmatine; protoberberine alkaloid; Enantia chlorantha
AbstractPalmatine and its reduced form, dl-tetrahydropalmatine are a group of isoquinoline alkaloids that have been reported to display a variety of biological and pharmacological activities. Both drugs are hydrophilic and are difficult to be purified by conventional purification methods of natural products. A high-performance displacement chromatography (HPDC) method successfully purified palmatine and its semi-synthetic derivative dl-tetrahydropalmatine from crude extract of the African medicinal plant Enantia chlorantha. The crude extract from the root bark of E. chlorantha was fractionated on an analytical reversed-phase C18 column by using 0.1% trifluoroacetic acid (TFA) or acetic acid/H2O as a carrier and cetylpyridinium trifluoroacetate (or acetate) (1.9 mg/mL) in 0.1% TFA (or acetic acid)/H2O as a displacer. Palmatine was quantitatively purified at >98% purity in the fully developed displacement mode. dl-Tetrahydropalmatine was semi-synthesized by NaBH4 reduction from crude palmatine and directly purified by HPDC. Both palmatine and dl-tetrahydropalmatine were identified by high-resolution electrospray tandem mass spectrometry, 1H NMR and 13C NMR. This is the first report of one-step HPDC purification of natural and semi-synthetic products from a complex crude extract.
Publication date
PublisherElsevier
LanguageEnglish
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedYes
NRC number47520
NPARC number21268255
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Record identifier3859ddb4-cdd2-4365-9648-cf57f4278c7c
Record created2013-06-10
Record modified2016-05-09
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