Bile salt-activated lipase expression during larval development in the haddock (Melanogrammus aeglefinus)

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DOIResolve DOI: http://doi.org/10.1016/j.aquaculture.2004.02.001
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TypeArticle
Journal titleAquaculture
Volume235
Pages601617; # of pages: 17
Subjectbile salt-activated lipase; fish larvae; gene expression; haddock; pancreas
AbstractIn the present work, we report the characterization of the partial cDNA sequence of a bile salt-activated lipase (BAL) cDNA from haddock. The predicted polypeptide encoded by the cDNA sequence contains the bile salt-binding site characteristic of all BALs at amino acid positions 36–45, and the lipid-binding site thus far only reported in fish BALs starting at position 345. Other features of BAL are also present including: the active site serine motif at positions 111–117, the catalytic triad formed by the residues S114, D239 and H358, and an N-glycosylation site at position 107. The relative levels of BAL gene expression were determined in haddock larvae during ontogeny by reverse transcription–polymerase chain reaction (RT–PCR) with the earliest detectable transcript levels identified at hatch. Using in situ hybridization, the BAL transcripts were localized consistently in the pancreas of haddock larvae from mouth opening until 401 degree days (DD). Using biochemical techniques, the specific activity of BAL was found to decline significantly over time. Our results also suggest that haddock larvae are capable of digesting lipids at the time of mouth opening.
Publication date
PublisherElsevier
Copyright noticeCrown Copyright D 2004 Published by Elsevier B.V. All rights reserved.
LanguageEnglish
AffiliationNational Research Council Canada; NRC Institute for Marine Biosciences; Human Health Therapeutics; Industrial Research Assistance Program
Peer reviewedYes
NRC number42432
1329
NPARC number3538113
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Record identifier38f8d279-64ae-46c3-991f-db091b1c20f4
Record created2009-03-01
Record modified2016-05-09
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