Single-domain antibody-nanoparticles : Promising architecturres for increased Staphylococcus aureus detection specificity and sensitivity

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DOIResolve DOI: http://doi.org/10.1021/bc900332r
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TypeArticle
Journal titleBioconjugate Chemistry
Volume20
Issue10
Pages19661974; # of pages: 9
Subjectnanoparticles; biomolecules; antibody-nanoparticle; nanoconjugates; biodiagnostics; superparamagnetic
AbstractBecause antibodies are highly target-specific and nanoparticles possess diverse, material-dependent properties that can be exploited in order to label and potentially identify biomolecules, the development of antibody-nanoparticle conjugates (nanoconjugates) has huge potential in biodiagnostics. Here, we describe a novel superparamagnetic nanoconjugate, one whose recognition component is a single-domain antibody. It is highly active toward its target Staphylococcus aureus, displays long shelf life, lacks cross-reactivity inherent to traditional homologue whole antibodies, and captures a few dozen S. aureus cells in a mixed cell population with ∼100% efficiency and specificity. We ascribe the excellent performance of our nanoconjugate to its single-domain antibody component and recommend it as a general purpose recognition element.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Biological Sciences; NRC Steacie Institute for Molecular Sciences; NRC Institute for Microstructural Sciences
Peer reviewedYes
NPARC number15295296
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Record identifier39a496cb-f0ce-409c-b13a-56c3ebf1eb03
Record created2010-05-17
Record modified2016-05-09
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