Effect of the HP0159 ORF mutation on the lipopolysaccharide structure and colonizing ability of Helicobacter pylori

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DOIResolve DOI: http://doi.org/10.1111/j.1574-695X.2008.00416.x
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TypeArticle
Journal titleFEMS Immunology & Medical Microbiology
Volume53
Issue2
Pages204213; # of pages: 10
SubjectHelicobacter pylori; lipopolysaccharide; structure; colonization
AbstractThe outer core region of Helicobacter pylori lipopolysaccharide of the majority of isolates contains an a-1,6-glucan polymer synthesized by the product of the HP0159 ORF. Structural studies carried out on HP0159 lipopolysaccharide mutants by a combination of chemical methods, mass spectrometry and nuclear magnetic resonance spectroscopy confirmed that insertional inactivation of HP0159 gene in H. pylori strains 26695 and SS1 resulted in formation of a truncated lipopolysaccharide molecule characterized by the presence of a terminal DD-heptose residue in the side-chain outer core fragment and maintaining an inner core backbone structure compared with the wild-type Lewis antigen-expressing strains. Colonization studies with HP0159 mutants of two mouse-colonizing strains, SS1 and M6, confirmed their inability to successfully colonize the murine stomach.
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberALTMAN2008A
NPARC number9362157
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Record identifier3a65e533-129e-488d-ba8f-96b89270327f
Record created2009-07-10
Record modified2016-05-09
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