Manufacturing of adenovirus vectors : production and purification of helper dependent adenovirus

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TypeBook Chapter
Book titleViral Vectors for Gene Therapy
Series titleMethods in Molecular Biology; Volume 737
Pages139156; # of pages: 18
AbstractAdenoviral vector (AdV) of the third generation also known as helper-dependent adenoviral vector (HDV) is an attractive delivery system for gene therapy applications. However, obtaining high quality-grade HDV in sufficient amount remains a challenge that hampers the extensive use of this vector in preclinical and clinical studies. Here we review recent progress in the large-scale manufacturing of HDV. The production of HDV is now amenable to large-scale volume with reduced process duration under optimized rescue and co-infection conditions. Also, efficient downstream processing of HDV with acceptable recovery of HDV and minimal contamination by the helper virus is described.
Publication date
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedYes
NRC numberCPBIO18
NPARC number21268260
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Record identifier3a871bb3-243f-42e0-82b6-7ffb068db3fd
Record created2013-06-11
Record modified2016-05-09
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