Mesophilic Aeromonas UDP-glucose pyrophosphorylase (GalU) mutants show two types of lipopolysaccharide structures and reduced virulence

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TypeArticle
Journal titleMicrobiology
Volume153
IssuePt 8
Pages23932404; # of pages: 12
SubjectACID; Aeromonas; Aeromonas hydrophila; Animals; BAND; biosynthesis; Blood Bactericidal Activity; Carbohydrate Sequence; Cell Adhesion; Cell Line,Tumor; chemistry; DIFFERENCE; DNA; DNA,Bacterial; enzymology; Fish Diseases; Fishes; Galactose; GENE; Genetic Complementation Test; genetics; Glucose; Gram-Negative Bacterial Infections; Humans; KDO; Lethal Dose 50; lipid; Lipid A; LIPID-A; LIPOPOLYSACCHARIDE; Lipopolysaccharides; LPS; Mice; Microbial Viability; Microbiology; MODEL; Molecular Sequence Data; MUTANT; MUTANTS; Mutation; O antigen; O Antigens; O-antigen; O1; pathogenicity; phosphate; physiology; RESIDUES; RESTRICTED; Sepsis; Sequence Analysis,DNA; SEROTYPE; SEROTYPES; Serum; STRAIN; STRAINS; structure; Synthesis; UNIT; UTP-Glucose-1-Phosphate Uridylyltransferase; veterinary; Virulence
AbstractA mutation in galU that causes the lack of O34-antigen lipopolysaccharide (LPS) in Aeromonas hydrophila strain AH-3 was identified. It was proved that A. hydrophila GalU is a UDP-glucose pyrophosphorylase responsible for synthesis of UDP-glucose from glucose 1-phosphate and UTP. The galU mutant from this strain showed two types of LPS structures, represented by two bands on LPS gels. The first one (slow-migrating band in gels) corresponds to a rough strain having the complete core, with two significant differences: it lacks the terminal galactose residue from the LPS-core and 4-amino-4-deoxyarabinose residues from phosphate groups in lipid A. The second one (fast-migrating band in gels) corresponds to a deeply truncated structure with the LPS-core restricted to one 3-deoxy-d-manno-oct-2-ulosonic acid (Kdo) and three l-glycero-d-manno-heptose residues. galU mutants in several motile mesophilic Aeromonas strains from serotypes O1, O2, O11, O18, O21 and O44 were also devoid of the O-antigen LPS. The galU mutation reduced to less than 1 % the survival of these Aeromonas strains in serum, decreased the ability of these strains to adhere and reduced by 1.5 or 2 log units the virulence of Aeromonas serotype O34 strains in a septicaemia model in either fish or mice. All the changes observed in the galU mutants were rescued by the introduction of the corresponding single wild-type gene
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberVILCHES2007
NPARC number9360808
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Record identifier3b47e912-a07d-4cd5-ac54-0066200f445d
Record created2009-07-10
Record modified2016-05-09
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