Haemoglobin-induced oxidative stress is associated with both endogenous peroxidase activity and H 2O 2 generation from polyunsaturated fatty acids

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DOIResolve DOI: http://doi.org/10.3109/10715762.2010.532492
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TypeArticle
Journal titleFree Radical Research
ISSN1071-5762
Volume45
Issue3
Pages303316; # of pages: 14
Subjectcatalase; hemoglobin; hydrogen peroxide; intercellular adhesion molecule 1; linoleic acid; low density lipoprotein; peroxidase; polyunsaturated fatty acid; probucol; reactive oxygen metabolite; vascular cell adhesion molecule 1; article; atherogenesis; cardiovascular disease; cell membrane; controlled study; human; human cell; incidence; lipid peroxidation; mortality; oxidative stress; protein expression; Anemia, Hemolytic; Antioxidants; Catalase; Endothelial Cells; Fatty Acids, Unsaturated; Hemoglobins; Humans; Hydrogen Peroxide; Intercellular Adhesion Molecule-1; Linoleic Acid; Lipoproteins, LDL; Membrane Lipids; Oxidation-Reduction; Oxidative Stress; Peroxidase; Probucol; Reactive Oxygen Species; Sodium Azide; Vascular Cell Adhesion Molecule-1
AbstractPatients with increased haemolytic haemoglobin (Hb) have 10-20-times greater incidence of cardiovascular mortality. The objective of this study was to evaluate the role of Hb peroxidase activity in LDL oxidation. The role of Hb in lipid peroxidation, H 2O 2 generation and intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) was assessed using NaN3, a peroxidase inhibitor, catalase, a H 2O 2 decomposing enzyme and human umbilical vein endothelial cells (HUVECs), respectively. Hb induced H 2O 2 production by reacting with LDL, linoleate and cell membrane lipid extracts. Hb-induced LDL oxidation was inhibited by NaN3 and catalase. Furthermore, Hb stimulated ICAM-1 and VCAM-1 expression, which was inhibited by the antioxidant, probucol. Thus, the present study suggests that the peroxidase activity of Hb produces atherogenic, oxidized LDL and oxidized polyunsaturated fatty acids (PUFAs) in the cell membrane and reactive oxygen species (ROS) formation mediated Hb-induced ICAM-1 and VCAM-1 expression. © 2011 Informa UK, Ltd.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Biological Sciences (IBS-ISB)
Peer reviewedYes
NPARC number21271470
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Record identifier3b78ae26-0248-480c-b8bd-6249954c274e
Record created2014-03-24
Record modified2016-05-09
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