Molecular and chemical characterization of blood cells by infrared spectroscopy : a new optical tool in hematology

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DOIResolve DOI: http://doi.org/10.1016/j.bcmd.2005.06.009
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TypeArticle
Journal titleBlood Cells, Molecules & Diseases
ISSN1079-9796
Volume35
Issue3
Pages404412; # of pages: 9
SubjectInfrared spectroscopy; Leukocytes; Erythrocytes; Platelets; Diagnosis
AbstractInfrared (IR) spectroscopy has made important contributions to the arena of hematology in the past decade. The normal physiology and pathologic modifications of the three cellular elements in blood, i.e., leukocytes, erythrocytes and platelets, have been thoroughly investigated by this recently emerged optical tool. By revealing subtle alterations in the structures of macromolecules in these blood cells, IR spectroscopy has become an ideal complementary analytical tool to conventional biochemical assays used to diagnose various common hematological disorders. Such traditional assays include molecular structure measurements that determine erythrocyte membrane fluidity and conformational changes, lipid profiling of platelet membranes, as well as assays of leukocyte proliferation and differentiation. IR spectroscopic-based techniques can be used to analyze DNA alterations, secondary structural changes in proteins, and to profile cellular lipids. From a molecular and biomedical perspective, IR spectroscopy has been explored for the diagnosis and prognosis of leukemia and hthalassemia, to predict drug sensitivity and resistance in chemotherapy patients, and more recently to examine apoptotic processes in blood cells. These studies have shown great promise in the early identification of drug-resistant patients and the early diagnosis of hematological disorders, especially malignancies. Furthermore, IR spectroscopic-based investigations will enable specific mechanisms underlying hematological disorders to be elucidated by revealing the molecular changes in the blood cells at a very early pathogenesis stage.
Publication date
PublisherElsevier Inc.
LanguageEnglish
AffiliationNRC Institute for Biodiagnostics; National Research Council Canada
Peer reviewedYes
NRC number2228
NPARC number9148515
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Record identifier3be37511-4a51-4dcb-92f8-22badfdfaae4
Record created2009-06-25
Record modified2016-08-12
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