Tetrameric repeat units associated with virulence factor phase variation in Haemophilus also occur in Neisseria spp. and Moraxella catarrhalis

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TypeArticle
Journal titleFEMS Microbiology Letters
Volume137
Issue1
Pages109114; # of pages: 6
SubjectBase Sequence; DNA Primers; DNA,Bacterial; Gene Expression; Haemophilus; Haemophilus influenzae; Human; LIPOPOLYSACCHARIDE; Lipopolysaccharides; Microsatellite Repeats; Molecular Sequence Data; Moraxella (Branhamella) catarrhalis; Neisseria; Neisseria meningitidis; Oligonucleotide Probes; Polymerase Chain Reaction; Support,Non-U.S.Gov't; Support,U.S.Gov't,Non-P.H.S.; Virulence
AbstractThe tetrameric repeat units 5'-CAAT-3' and 5'-GCAA-3' are associated with phase variable expression of lipopolysaccharide biosynthetic genes in Haemophilus influenzae. Four other tetrameric repeat units have also been reported from H. influenzae strain Rd, 5'-CAAC-3', 5'-GACA-3', 5'-AGCT-3', and 5'-TTTA-3', which are also associated with putative virulence factors. Using oligonucleotide probes corresponding to five tandem copies of each of these tetramers, we have screened three strains of Neisseria meningitidis and one each of Neisseria gonorrhoeae, Neisseria lactamica, Haemophilus parainfluenzae, Bordetella pertussis, Bordetella parapertussis, Bordetella bronchiceptica and Moraxella catarrhalis for the presence of these motifs. We have demonstrated the presence of multiple copies of the 5'-GCAA-3' motif in all the Neisseria strains tested, and also the repeated motif 5'-CAAC-3' in M. catarrhalis. We have further demonstrated by Southern blot analysis that the 5'-CAAC-3' repeats detected in M. catarrhalis are probably associated with the same genes as in H. influenzae, but that the 5'-GCAA-3' motifs in N. meningitidis are not. The use of characterised tetrameric DNA sequences as hybridisation probes may prove useful in the identification of novel phase variable virulence determinants in organisms other than H. influenzae Type: JOURNAL ARTICLE Language: Eng
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberPEAK1996
NPARC number9388757
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Record identifier3c20fc82-4dac-48ad-83de-c7bb336da6bb
Record created2009-07-10
Record modified2016-05-09
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