The effect of aliphatic alcohols on fluid bilayers in unilamellar DOPC vesicles — A small-angle neutron scattering and molecular dynamics study

Download
  1. (PDF, 886 KB)
  2. Get@NRC: The effect of aliphatic alcohols on fluid bilayers in unilamellar DOPC vesicles — A small-angle neutron scattering and molecular dynamics study (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1016/j.bbamem.2011.04.010
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleBiochimica et Biophysica Acta
Volume1808
Issue9
Pages21362146; # of pages: 11
AbstractSmall-angle neutron scattering and coarse-grained molecular dynamics simulations have been used to determine the structural parameters (bilayer thickness D, polar region thickness DH, interfacial lateral area of the unit cell AUC and alcohol partial interfacial area ACnOH) of fluid dioleoylphosphatidylcholine:dioleoylphosphatidylserine (PCPS, DOPC:DOPS = 24.7 mol:mol) bilayers in extruded unilamellar vesicles with incorporated aliphatic alcohols (CnOH, n = 8–18 is the even number of carbons in alkyl chain). External 2H2O/H2O contrast variation experiments revealed that DH decreases as a function of alkyl chain length and CnOH:PCPS molar ratio. Using measurements at single 100% 2H2O contrast we found that (i) D decreases with CnOH:PCPS molar ratio and increases with CnOH chain length (at 0.4 molar ratio); (ii) AUC significantly increases already in the presence of shortest CnOH studied (at 0.4 molar ratio), further increase is observed with longer CnOHs and at higher molar ratios; (iii) ACnOH of alcohol molecules in PCPS bilayer increases linearly with the alkyl chain length, ACnOH obtained for CnOHs with n ≤ 10 corresponds to ACnOH ≤ 20Å2 — a value specific for the crystalline or solid rotator phase of alkanes. All these structural modifications induced by studied CnOHs were reproduced in MD simulations. The computational results give an accurate description of the alcohol effects at the molecular level, explaining the experimental data. The anomaly in ACnOH is discussed via the “umbrella” effect described for cholesterol.
Publication date
LanguageEnglish
AffiliationNRC Canadian Neutron Beam Centre; National Research Council Canada
Peer reviewedYes
NPARC number18749427
Export citationExport as RIS
Report a correctionReport a correction
Record identifier3cd64f7f-196a-4c8c-8a3c-d5b80a3bcebb
Record created2011-11-10
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)
Date modified: