Carbohydrate functionalization of silver nanoparticles modulates cytotoxicity and cellular uptake

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DOIResolve DOI: http://doi.org/10.1186/s12951-014-0059-z
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TypeArticle
Journal titleJournal of Nanobiotechnology
ISSN1477-3155
Volume12
Issue1
Article number59
SubjectCarbonylation; Ethylene; Nanoparticles; Polyols; Toxicity; Biointerfaces; Carbohydrate coatings; Functionalizations; Nanotoxicology; Silver; carbohydrate; ethylene glycol; galactose; glucose; mannose; monosaccharide; reactive oxygen metabolite; silver nanoparticle; sugar; carbohydrate analysis; cell culture; cell surface; cellular parameters; cellular uptake; confocal microscopy; cytotoxicity; liver cell; MTT assay; oxidative stress; particle size; polyacrylamide gel electrophoresis; protein carbonylation; roentgen spectroscopy; synthesis; transmission electron microscopy; zeta potential
AbstractBackground: Increasing use of silver nanoparticles (Ag-NPs) in various products is resulting in a greater likelihood of human exposure to these materials. Nevertheless, little is still known about the influence of carbohydrates on the toxicity and cellular uptake of nanoparticles. Methods: Ag-NPs functionalized with three different monosaccharides and ethylene glycol were synthesized and characterised. Oxidative stress and toxicity was evaluated by protein carbonylation and MTT assay, respectively. Cellular uptake was evaluated by confocal microscopy and ICP-MS. Results: Ag-NPs coated with galactose and mannose were considerably less toxic to neuronal-like cells and hepatocytes compared to particles functionalized by glucose, ethylene glycol or citrate. Toxicity correlated to oxidative stress but not to cellular uptake. Conclusions: Carbohydrate coating on silver nanoparticles modulates both oxidative stress and cellular uptake, but mainly the first has an impact on toxicity. These findings provide new perspectives on modulating the bioactivity of Ag-NPs by using carbohydrates.
Publication date
PublisherBioMed Central
LanguageEnglish
AffiliationNational Research Council Canada; Measurement Science and Standards
Peer reviewedYes
NPARC number21275453
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Record identifier3e93f58d-2ab6-47c7-8617-08a5f9183303
Record created2015-07-14
Record modified2016-05-09
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