Prototype single step lateral flow technology for detection of avian influenza virus and chicken antibody to avian influenza virus

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DOIResolve DOI: http://doi.org/10.1080/15321810701603443
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TypeArticle
Journal titleJournal Of Immunoassay & Immunochemistry
Volume28
Issue4
Pages307318; # of pages: 12
SubjectLateral flow technology, Avian influenza virus, Chicken antibody
AbstractA rapid and effective lateral flow assay (LFA) for detection of avian influenza virus (AIV) was developed. For antigen capture, the assay used monoclonal antibody specific for a conserved nuclear protein (NP) epitope, immobilized on a cellulose acetate matrix, in conjunction with a second NP monoclonal antibody chemically linked to either coloured latex beads or colloidal gold particles contained in a sample pad for detection. Virus sample added to the sample pad flowed into the trapping antibody to form a visible band as well as a second, control band further along the acetate strip. The control band consisted of recombinant protein A/G, also immobilized on the matrix. A second LFA for detection of chicken antibody to AIV was developed where NP antigen was immobilized on the matrix with recombinant protein A/G immobilized as a control band. Latex beads or colloidal gold particles to which monoclonal anti-chicken antibody was attached, were used as the indicator system.
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedYes
NRC numberNIELSEN2007
NPARC number9379036
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Record identifier3f19441b-c629-46bd-bccc-0d34eba0f416
Record created2009-07-10
Record modified2016-06-01
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