Microbial resolution of baclofen by a new isolate of streptomyces halstedii

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DOIResolve DOI: http://doi.org/10.1016/S1389-1723(02)80237-7
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TypeArticle
Journal titleJournal of Bioscience and Bioengineering
ISSN1389-1723
Volume93
Issue6
Pages557562; # of pages: 6
Subject(R)-baclofen; bio; Streptomyces halstedii; resolution
AbstractWe attempted to isolate microorganisms able to metabolize (S)-baclofen while showing little or no activity with (R)-baclofen. A culture collection of 218 microbial isolates was established from several soil samples using various approaches. Subseqnent chiral HPLC analysis revealed that only two cultures showed promise. Initial shake flask experiments demonstrated a correlation between culture growth and (S)-baclofen metabolism. (S)-baclofen was metabolized much more rapidly than (R)-balcofen. The best culture, identified as Streptomyces halstedii, was used to scale up the process in small-scale fed-batch fermentations using pulse addition of racemic baclofen to yield extracellular fractions showing enantiomeric excess (e.e.) values of greater than 90% in favour of the (R)-isomer. Resting cells also were able to carry out the wanted reaction but initial attempts using cell free extracts were negative. It is speculated that the initial attack on (S)-baclofen may be catalyzed by a mono- or dioxygenase. (author abst.)
Publication date
AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Peer reviewedNo
Identifier18619782
NRC number37700
NPARC number12329150
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Record identifier415518f3-35b3-4b1f-814f-9177e3368206
Record created2009-09-10
Record modified2016-05-09
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