Structural studies of the cell wall polysaccharides from three strains of Lactobacillus helveticus with different autolytic properties: DPC4571, BROI, and LH1

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DOIResolve DOI: http://doi.org/10.1016/j.carres.2013.05.020
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TypeArticle
Journal titleCarbohydrate Research
ISSN0008-6215
Volume379
Pages712; # of pages: 6
Subject2D-NMR spectroscopy; Autolytic properties; Cell wall polysaccharides; Cell-wall components; Intracellular enzyme; Lactobacillus helveticus; Methylation analysis; Structural studies; Alkylation; Antigen-antibody reactions; Cells; Enzymatic hydrolysis; Nuclear magnetic resonance spectroscopy; Polysaccharides; Strain; Structure (composition); Cytology; autolysin; peptidoglycan; polysaccharide; teichoic acid; article; autolysis; bacterial strain; cell wall; chemical structure; hydrolysis; Lactobacillus helveticus; methylation; nonhuman; nuclear magnetic resonance spectroscopy; priority journal; protein structure; Lactobacillus helveticus
AbstractLactobacillus helveticus is traditionally used in dairy industry as a starter or an adjunct culture for manufacture of cheese and some types of fermented milk. Its autolysis releases intracellular enzymes which is a prerequisite for optimum cheese maturation, and is known to be strain dependent. Autolysis is caused by an enzymatic hydrolysis of the cell wall peptidoglycan (PG) by endogenous peptidoglycan hydrolases (PGHs) or autolysins. Origins of differences in autolytic properties of different strains are not fully elucidated. Regulation of autolysis possibly depends on the structure of the cell wall components other than PG, particularly polysaccharides. In the present work, we screened six L. helveticus strains with different autolytic properties: DPC4571, BROI and LH1. We established, for the first time, that cell walls (CWs) of these strains contained polysaccharides, different from their CW teichoic acids. Cell wall polysaccharides of three strains were purified, and their chemical structures were established by 2D NMR spectroscopy and methylation analysis. The structures of their repeating units are presented. © 2013 Elsevier Ltd.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC)
Peer reviewedYes
NPARC number21270591
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Record identifier41b1fb8c-f014-4077-8ab1-a34915352453
Record created2014-02-17
Record modified2016-05-09
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