Structure of the O-chain of the lipopolysaccharide of Haemophilus pleuropneumoniae serotype 1: Biochem.Cell Biol.

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TypeArticle
Journal titleBiochem.Cell Biol.
Volume64
Issue12
Pages13171325; # of pages: 9
Subject13C; 1H; Acetylation; ACID; analysis; bacterial; Carbohydrate Sequence; Deamination; Electrophoresis; Haemophilus; Hydrolysis; lipid; Lipid A; LIPID-A; LIPOPOLYSACCHARIDE; Lipopolysaccharides; MAGNETIC; Magnetic Resonance Spectroscopy; MAGNETIC-RESONANCE; Methylation; MOLECULAR; Molecular Weight; NUCLEAR; Nuclear Magnetic Resonance; NUCLEAR-MAGNETIC-RESONANCE; O-chain; Oxidation-Reduction; Periodic Acid; Pleuropneumoniae; POLYSACCHARIDE; Polysaccharides; Polysaccharides,Bacterial; REPEATING UNIT; RESONANCE; SEROTYPE; Sodium; structure; UNIT; yield
AbstractBy phenol-water extraction an aqueous-phase soluble cellular lipopolysaccharide was isolated from Haemophilus pleuro-pneumoniae serotype 1. It was shown by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, hydrolysis, methylation, and both one- and two-dimensional 1H and 13C nuclear magnetic resonance studies to be an S-type lipopolysaccharide, which could be cleaved to yield a lipid A and an O-chain polysaccharide identified as a high molecular weight branched polymer of a tetrasaccharide repeating unit having the structure: (Formula: see text)
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberALTMAN1986B
NPARC number9361488
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Record identifier427a63c8-e0e7-4568-bcb8-46072a3c6598
Record created2009-07-10
Record modified2016-05-09
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