Biomolecule delivery into canola protoplasts by centrifuging cells with microbubbles

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DOIResolve DOI: http://doi.org/10.1016/j.febslet.2012.12.005
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TypeArticle
Journal titleFEBS Letters
ISSN0014-5793
Volume587
Issue3
Pages285290; # of pages: 6
Subjectfluorescein isothiocyanate dextran; cell membrane; cell nucleus; cell transport; cell viability; cell wall; confocal microscopy; cytoplasm; flow cytometry; microbubble; protoplast; Biological Transport; Brassica; Cell Survival; Centrifugation; Cytological Techniques; Diffusion; Drug Delivery Systems; Fluorescein-5-isothiocyanate; Microbubbles; Protoplasts; Brassica napus var. napus
AbstractWe have successfully delivered FITC and FITC-Dextran (70, 250 kDa) into canola protoplasts by centrifuging cells with different amounts of microbubbles at variable centrifuge speed. The efficiency is around 90%, while cell viability remains high. Confocal microscopy images show that both FITC and FITC-Dextran are scattered inside the cytoplasm and the cell nucleus. Pores are observed on canola protoplast cell membranes and cell walls when centrifuged with microbubbles, while the membrane of cells centrifuged alone remain intact and smooth. We hypothesize that the collision between the microbubbles and cells or the bursting of microbubbles are the main reasons for the formation of these pores. Biomaterials can diffuse into the cells once the pathway is created. © 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); Security and Disruptive Technologies
Peer reviewedYes
NPARC number21271799
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Record identifier433c6466-34b6-4a4a-a97a-6acb3e9827b2
Record created2014-04-22
Record modified2016-05-09
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