Protein storage vacuoles of Brassica napus zygotic embryos accumulate a BURP domain protein and perturbation of its production distorts the PSV

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DOIResolve DOI: http://doi.org/10.1007/s11103-009-9541-7
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TypeArticle
Journal titlePlant Molecular Biology
Volume71
Issue4-5
Pages331343; # of pages: 13
SubjectBNM2; BURP domain protein; Canola; Post transcriptional gene control; Protein storage vacuoles; Seed-specific protein
AbstractBNM2 is a prototypical member of the enigmatic BURP domain protein family whose members contain the signature FX6–7GX10–28PX25–31CX11–12X2SX45–56CHX10CHX25–29CHX2TX15–16PX5CH in the C-terminus. This protein family occurs only in plants, and the cognate genes vary very widely in their expression contexts in vegetative and reproductive tissues. None of the BURP family members has been assigned any biochemical function. BNM2 was originally discovered as a gene expressed in microspore-derived embryos (MDE) of Brassica napus but we found that MDE do not contain the corresponding protein. We show that BNM2 protein production is confined to the seeds and localized to the protein storage vacuoles (PSV) even though the transcript is found in vegetative parts and floral buds as well. In developing seeds, transcript accumulation precedes protein appearance by more than 18 days. RNA accumulation peaks at ~20 days post anthesis (DPA) whereas protein accumulation reaches its maximum at ~40 DPA. Transgenic expression of BNM2 does not abrogate this regulation to yield ectopic protein production or to alter the temporal aspect of BNM2 accumulation. Overexpression of BNM2 led to spatial distortion of storage protein accumulation within PSV and to some morphological alterations of PSVs. However, the overall storage protein content was not altered.
Publication date
LanguageEnglish
AffiliationNRC Plant Biotechnology Institute; National Research Council Canada
Peer reviewedYes
NRC number50148
NPARC number19078542
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Record identifier437e96b0-23f5-449a-bf85-2ce5f27e1e13
Record created2012-03-30
Record modified2016-05-09
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