Tocopherol activity correlates with its location in a membrane: A new perspective on the antioxidant vitamin e

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DOIResolve DOI: http://doi.org/10.1021/ja312665r
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TypeArticle
Journal titleJournal of the American Chemical Society
ISSN0002-7863
Volume135
Issue20
Pages75237533; # of pages: 11
SubjectAntioxidant mechanisms; Hydrocarbon matrix; Hydrophobic-hydrophilic; Lipid acyl-chains; Measurements of; Oxidation kinetics; Physical locations; Reactive oxygen species; Cytology; Lipid bilayers; Neutron diffraction; Ultraviolet spectroscopy; Physical addresses; alpha tocopherol; hydrocarbon; hydrogen; hydroxyl group; reactive oxygen metabolite; tocopherol; antioxidant activity; hydration; hydrophilicity; hydrophobicity; lipid bilayer; lipid membrane; lipid oxidation; membrane structure; neutron diffraction; nuclear magnetic resonance spectroscopy; review; ultraviolet spectroscopy
AbstractWe show evidence of an antioxidant mechanism for vitamin E which correlates strongly with its physical location in a model lipid bilayer. These data address the overlooked problem of the physical distance between the vitamin's reducing hydrogen and lipid acyl chain radicals. Our combined data from neutron diffraction, NMR, and UV spectroscopy experiments all suggest that reduction of reactive oxygen species and lipid radicals occurs specifically at the membrane's hydrophobic-hydrophilic interface. The latter is possible when the acyl chain "snorkels" to the interface from the hydrocarbon matrix. Moreover, not all model lipids are equal in this regard, as indicated by the small differences in vitamin's location. The present result is a clear example of the importance of lipid diversity in controlling the dynamic structural properties of biological membranes. Importantly, our results suggest that measurements of aToc oxidation kinetics, and its products, should be revisited by taking into consideration the physical properties of the membrane in which the vitamin resides. © 2013 American Chemical Society.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Canadian Neutron Beam Centre (CNBC-CCFN)
Peer reviewedYes
NPARC number21269847
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Record identifier439a0a03-56d5-4ac9-981f-7ec9500f26a3
Record created2013-12-13
Record modified2016-05-09
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