Enhancing caries resistance in occlusal fissures with a short-pulsed CO 2 9.6 μm laser - An in vitro pH-cycling study - Preliminary results

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DOIResolve DOI: http://doi.org/10.1117/12.881705
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TypeArticle
Proceedings titleProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ConferenceLasers in Dentistry XVII, 23 January 2011 through 23 January 2011, San Francisco, CA
ISSN1605-7422
ISBN9780819484215
Volume7884
Article number78840G
SubjectCO <sub>2</sub> laser; In-vitro; Microsecond pulsed; Optical coherence Tomography; Polarized Rama spectroscopy; Quantitative light-induced fluorescence; SoproLife light-induced fluorescence; Dentistry; Extraction; Fluorescence; Hardness; Hardness testing; Porphyrins; Pulse repetition rate; Raman spectroscopy; Surface properties; Tomography; Pulsed lasers
AbstractTreatment of occlusal surfaces with a short-pulsed CO 2 9.6 μm wavelength laser has previously been proposed as a method for caries prevention. A sample of 20 extracted human molars were measured before and after demineralizationremineralization pH-cycling with ICDAS II visual inspection, DIAGNOdent, quantitative light-induced fluorescence (QLF), SoproLife in daylight and blue light-induced fluorescence mode, optical coherence tomography (OCT) and polarized Raman spectroscopy (PRS). Per tooth, one fissure was subjected to laser treatment using a short-pulsed CO 2 laser at 9.6 μm wavelength with a fluence of 3.5 J/cm 2, 20 Hz pulse repetition rate, 20 μs pulse duration, angulated handpiece, and focus diameter of 600 μm, while the other fissure was left untreated as control. The teeth were subjected to a demineralization-remineralization pH-cycling for 9 days. Cross-sectional micro-hardness testing was done as a gold standard to compare results with findings from the other detection methods used. Due to the small sample size reported, the trend observed was that laser treated fissures demonstrated a smaller relative mineral loss △Z than the controls. QLF findings followed a similar trend. Using a rotary catheter probe, OCT measurements were acquired from the various fissures to generate circularly mapped OCT depth images. PRS measurements of parallel- and cross-polarized spectra were acquired with a Raman microscope system. Preliminary OCT images showed differences in the initial air-tooth interface, with PRS results indicating a change in the surface property along with biochemical alterations after pH-cycling. Following pH-cycling, an increase in the OCT subsurface light backscattering intensity in the control fissures was observed compared to the laser test fissures. Porphyrin based fluorescence methods like DIAGNOdent and SoproLife, respectively demonstrated only additional light scattering due to the demineralization process. © 2011 SPIE.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Biodiagnostics (IBD-IBD)
Peer reviewedYes
NPARC number21271489
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Record identifier439de0a3-965e-4809-831a-23673cf3bcb3
Record created2014-03-24
Record modified2016-05-09
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