Protection of cortical neurons against oxygen-glucose deprivation and N-methyl-D-aspartate by DIDS and SITS

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TypeArticle
Journal titleEur J Pharmacol
Volume464
Issue1
Pages1725; # of pages: 9
Subject4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid; 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid; ACID; Anaerobiosis; Animals; Calcium; Canada; Cell Survival; Cells,Cultured; Cerebral Cortex; cytology; Dose-Response Relationship,Drug; drug effects; Female; Glucose; Glutamic Acid; Membrane Potentials; metabolism; N-Methylaspartate; Neurons; Oxygen; pharmacology; physiology; Pregnancy; Protection; Rats; Rats,Sprague-Dawley; receptor; Support,Non-U.S.Gov't; toxicity
AbstractThe Cl(-) channel blockers, 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) or 4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid (SITS) dose-dependently protected against oxygen-glucose deprivation in cultured rat cortical neurons. DIDS or SITS attenuated oxygen-glucose deprivation-induced increases in extracellular glutamate concentrations and intracellular Ca(2+). DIDS or SITS provided moderate protection against N-methyl-D-aspartate (NMDA) toxicity and decreased NMDA receptor-mediated increases in intracellular Ca(2+). Whole-cell NMDA receptor currents were attenuated 39+/-2% and 21+/-3% by 1 mM DIDS and SITS, respectively. Other Cl(-) channel blockers as equipotent as DIDS and SITS did not decrease oxygen-glucose deprivation- or NMDA-mediated neuronal Ca(2+) influx or toxicity. Neurotoxicity by exogenous glutamate was not prevented by SITS and was exacerbated by DIDS. Reductions in oxygen-glucose deprivation-induced increases in intracellular Ca(2+) levels underlie neuroprotection by DIDS and SITS. This was a reflection of lower extracellular [glutamate], direct inhibition of Ca(2+) influx through postsynaptic NMDA receptors, and possibly through other protective properties associated with DIDS and SITS
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Institute for Biological Sciences
Peer reviewedNo
NRC numberTAUSKELA2003A
NPARC number9382806
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Record identifier43a21616-c017-456d-b5fe-6ae04b5a2e81
Record created2009-07-10
Record modified2016-05-09
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