Inkjet application, chromatography, and mass spectrometry of sugars on nanostructured thin films

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DOIResolve DOI: http://doi.org/10.1007/s00216-013-7131-7
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TypeArticle
Journal titleAnalytical and Bioanalytical Chemistry
ISSN1618-2642
Volume405
Issue23
Pages71957203; # of pages: 9
SubjectDerivatizations; Electrospray ionization mass spectrometry; Glancing Angle Deposition; High performance thin layer chromatography; Miniaturized layers; Quantitation; Ultrathin-layer chromatographies; Vapor-deposition techniques; Deposition; Ink jet printers; Mass spectrometry; Physical vapor deposition; RNA; Sugars; Chromatography; Theobroma cacao
AbstractUltrathin-layer chromatography (UTLC) potentially offers faster analysis, reduced solvent and sample volumes, and lower costs. One novel technique for producing UTLC plates has been glancing angle deposition (GLAD), a physical vapor deposition technique capable of aligning macropores to produce interesting separation properties. To date, however, GLAD-UTLC plates have been restricted to model dye systems, rather than realistic analytes. This study demonstrates the transfer of high-performance thin-layer chromatography (HPTLC) sugar analysis methods to GLAD-UTLC plates using the office chromatography framework. A consumer inkjet printer was used to apply very sharp low volume (3-30 nL) bands of water-soluble analytes (lactose, sucrose, and fructose). Analytic performance measurements extrapolated the limits of detection to be 3-5 ng/zone, which was experimentally proven down to 60-70 ng/band, depending on the sugar. This qualitative analysis of sugars in a commercially available chocolate sample is the first reported application of GLAD-UTLC to food samples. The potential utility of GLAD-UTLC is further exemplified by successful coupling with electrospray ionization mass spectrometry for the first time to characterize underivatized sugars.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); Security and Disruptive Technologies
Peer reviewedYes
NPARC number21270525
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Record identifier4458f52c-9f80-4fac-b32a-95d35d2f8d5b
Record created2014-02-17
Record modified2016-05-09
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